Please use the protocol mentioned on page 14 of the manual when using the ibind flex western system in conjunction with the licor odyssey imaging system. Whatever your imaging application the sapphire biomolecular imager and the powerful sapphire capture software will deliver high quality quantifiable data. Staining of j774 lysate with human anti actin beta hca147 followed by horseradish peroxidase conjugated mouse anti streptag antibody, clone streptag ii staining of hela whole cell lysate with. Operators manual licor odyssey operators manual 29 pages. Ge typhoon 9410 microarray imager variable mode imager. However, the sapphire biomolecular imager is a next generation laser scanning system which offers the best of both worlds. Jun 12, 2008 the pearl imager joins li cor s line up of imaging systems for dna, small animal, and proteomic research including the odyssey imaging system and mousepod, which also offers researchers a costeffective approach to in vivo small animal imaging. Li cor odyssey is an infrared imaging system that is able to eliminate background fluorescence from samples which. Page 1 the safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. Human anti human actin beta antibody, clone abd12141 recognizes the 42 kda product of the actb gene, cytoplasmic actin beta, also known as actin cytoplasmic 1. Full western blot images are shown in supplementary.
Which is the best and more sensitive western blot imaging. Can be used to distinguish between normal and cancer cells. The availability of licor odyssey antibodies makes. All images are acquired using the li cor odyssey classic imager and associated image pro analysis software version 3. In addition, software functions related to the odyssey mousepod accessory are discussed in the. Computer software cables cords manual chemiluminescence and. Which is the best and more sensitive western blot imaging system. Licor odyssey 9120 imaging system the lab world group. The licor odyssey 9120 imaging system gives your lab clear sharp digital results without the background fluorescence. Licor aerius 9250 automated infrared imaging system. Scientific fluorescence scanner for gel and blot imaging. The odyssey infrared imaging system allows multiplex western blotting and accurate protein detection and quantification using secondary antibodies labeled with irdye nearinfrared nir fluorescent dyes. Licor, odyssey infrared imaging system detecting infrared excited and emitted signals high sn ratio from membranes of western blots, protein arrays, micorplates of incell western assays, oncell.
The li cor odyssey fc imaging system is easy to use and the operation is selfexplanatory. View and download li cor odyssey operators manual online. Shown with optional shaker the belly dancer, stovall life science inc. By using digital images, strong and weak bands are detected on the same. The li cor odyssey 9120 imaging system gives your lab clear sharp digital results without the background fluorescence. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual. Licor aerius 9250 automated infrared imaging system ebay. By using digital images, strong and weak bands are detected on the same blot, saving labs time by not waiting on film to develop or the uncertainty whether a weak band requires multiple exposures to confirm. Used for visualizing images of genes, gene fragments, and gene products. The licor odyssey system is a sensitive far rednear infrared twocolor fluorescence. The odyssey clx imager is intended to be used by qualified personnel. A guide to modern quantitative fluorescent western. Odyssey fcimager placementinthelaboratory theodysseyfcimagerweighsapproximately27kg60lbs.
Correct the raw incell elisa signal for the background signal by subtracting the mean signal of wells incubated in the absence of the primary antibody from all other readings. For measurements of gas exchange between terrain and the atmosphere, licor eddy covariance solutions combine precision instrument platforms, flux computation systems. Be aware of the hazards associated with any chemical before using it. Licor eddy covariance analyzers have the lowest cost configuration and power requirements among similar instruments. Prepare samples and determine protein concentrations 2. Licor biosciences introduces nearinfrared small animal. The licor odyssey scanner is recommended for two color fluorescence imaging of western blots and in cell westerns microplates in far red and near infrared channels.
The page provides a catalogue of brands and devices, each offering to view or. In addition, software functions related to the odyssey mousepod accessory are discussed in the odyssey in vivo imaging guide. Licor odyssey scanner certified with warranty for sale. Summary of contents for licor odyssey page 1 the safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. These manuals require a valid vin and mailing address.
The blots were washed, treated with a secondary antimouse alexa 680 nm conjugate molecular probes and imaged using a licor odyssey imager. Analyzing western blots with image studio lite february 02, 20 image studio lite is a free software package from li cor biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. Protein detection system is intended for research use only. The page provides a catalogue of brands and devices, each offering to view or download an updated manual. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel red detection 600 nm are available.
Our school recently purchased a western blot documentation system to start slowly moving away from film. The licor odyssey fc imaging system is easy to use and the operation is selfexplanatory. Fluorescence wstacker odyssey 9260 for sale biodirect usa. The safety of this product can not be guaranteed if the product is used in any other way than is specified in this manual. Image studio lite is a free software package from licor biosciences aimed at life scientists that want to analyze gels, western blots, dot. Quantitative gel and blot imaging and analysis shared. The pearl imager joins licors line up of imaging systems for dna, small animal, and proteomic research including the odyssey imaging system and mousepod, which also offers. The sapphire biomolecular imager quantitative western blots. The licor odyssey 9120 infrared imaging system passed all tests and was found to be fully functional. Actin beta is ubiquitously expressed in all eukaryotic cells, playing a critical role in cell survival, motility and remodelling. All steps were performed as described in the user manual of the kit. The sapphire biomolecular imager quantitative western.
Western blot detection support getting started thermo. Chemiluminescence similar sensitivity to film resolution. Page 6 streptavidin incubation wash scan blot on odyssey chapter 3 of the odyssey operators manual describes how to place the blot on the odyssey scanning surface. Printinghistory publicationnumber97716222 printedjune2016 globalheadquarters servingtheunitedstates4647superiorstreetlincoln,ne68504 tollfree. Odyssey clx infrared imaging system from licor biosciences. Blots were scanned by licor odyssey infrared imaging system and analyzed by image j 1.
The odyssey infrared imaging system allows multiplex western blotting and accurate protein detection and quantification using secondary antibodies labeled. The li cor odyssey 9120 infrared imaging system passed all tests and was found to be fully functional. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created. A guide to modern quantitative fluorescent western blotting. Instrument utilities and diagnostics user diagnostics if you have a problem with your odyssey imager, licor technical support staff may ask you to perform the user diagnostic function. Odyssey clx infrared imaging system operators manual. Information on odyssey software can be found both in this manual and in the odyssey user guide. Digital imaging accessories manuals and user guides all. A good place to start is to read the overview of odyssey in this chapter. Licor biosciences, clx operators manual, clx instructions manual, clx, clx instrument manual created date. Odyssey clx application and technical notes show the versatility of the clx. Odyssey fc manuals, technical notes, white papers, and. Manuals, software information, and webinars are also listed.
Ge lifesciences molecular dynamics ge healthcare typhoon 9410 variable mode imager unites proven storage phosphor autoradiography technology with fourcolor nonradioactive fluorescent labeling. Two different wavelengths for western blot fluorescent dyes 680 and 800nm and pcr gels dyed with gel. Imaging western blots and protein gels on the licor odyssey. Licor products have been designed to be safe when operated in the manner described in this manual. We have 1 odyssey licor manual available for free pdf download. Chapter 2 of the odyssey user guide describes how to start scans and set the scanning parameters. Electrophoresis membrane transfer blocking antibody addition detection sample prep snap i. Western blotting accessories licor blot washer configured for washing and secondary antibody incubation of four. The odyssey infrared imaging system weighs approximately 33 kg 72 lb. Western blotting accessories licor blot washer configured for washing and secondary antibody incubation of four blots in incubation boxes. If you suspect a problem with the instrument, the user diagnostic can also be used to verify a problem before contacting technical support.
The odyssey fc imager is intended to be used by qualified personnel. A multi user site license for imagequant tl iqtl is available for the analysi sof typhoon data. Western blotting wb is an analytical technique originally developed in the late 1970s to determine the presence or absence of a protein of interest in a complex biological sample, such as a tissue. The safety of this product can not be guaranteed if the product is used in any other way than is. Anti human actin beta antibody, clone abd12141 biorad. All users are responsible for the proper use, according to defined protocol, when using the li.
All users are responsible for obtaining the proper approval and training before the use of the li. Transfer to a membrane licor, protein electrotransfer methods 4. Used licor odyssey 9120 digital imaging system for sale. Imaging western blots and protein gels on the licor. Prepare samples and determine protein concentrations.
Gel and blot imaging shared resources fred hutchinson. We demod the licor odyssey, biorad chemidoc, and the syngene pxi. The odyssey imager should not be used with any radioactive materials. Gpi tm150bp manuals and user guides, measuring instruments. Does anybody have experience with blocking reagents. The availability of licor odyssey antibodies makes detection of multiple proteins very easy. Odyssey digital imaging accessories pdf manual download. We have 1 licor odyssey manual available for free pdf download. Tested connection to software through tplink switch.
Ive used it in multiple applications and have noticed a higher quality output compared to traditional techniques. Licor biosciences, odyssey infrared imaging system b446. Used other licor odyssey imager for sale dotmed listing. The li cor odyssey system is a sensitive far rednear infrared twocolor fluorescence scanner for the imaging and quantitation of gels, blots, 96well plates and a variety of other applications, including tissues and whole animals. Licor odyssey is an infrared imaging system that is able to eliminate background fluorescence from samples which. I have been working with licor odyssey for last 45 years nowand what i observe is that licor odyssey blocking buffer or licor casein blocking buffer works best and you see less background. Featuring miniaturized electronics and simplified hardware, the newlyredesigned li7500ds open path co 2 h 2 o analyzer requires only 4 watts of power during normal operationlower than any other ec gas analyzer available.
Mar 10, 2015 the blots were washed, treated with a secondary antimouse alexa 680 nm conjugate molecular probes and imaged using a li cor odyssey imager. Licor, odyssey infrared imaging system detecting infrared excited and emitted signals high sn ratio from membranes of western blots, protein arrays, micorplates of incell western assays, oncell western assays, gels of dna, protein, coomassiestained, tissue section imaging,whole organ imaging, small animal imaging, elisas. Measure using a licor odyssey scanner in the 700 nm channel or measure od at 595 nm in a standard microplate spectrophotometer. Operators manual odyssey licor operators manual 2 pages. Licor biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be followed at all times. Western system in conjunction with the licor odyssey. Nrb common equipment neuroscience suny upstate medical. Licor odyssey clx infared imaging system enables twocolor detection and quantification of fluorescent dyelabeled antibodies room 3721 lonza 4dnucleofector, core, xunit, yunit enables highly efficient. Licor odyssey clx amersham typhoon nir fluorescence.
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